The Spectrophotometer is widely known as an analytical instrument, which can quantitatively measure the conduction or reflection of UV light, visible light and infrared light. This assists in the measurement of substance concentration such as DNA or RNA, protein, enzymatic reactions and development of bacterial cells. Spectrophotometer is an exclusive device made for measuring the wavelengths of light in a wide electromagnetic spectrum. This assists in the spectroscopic analysis of many sample materials. Most remarkable thing about the spectrophotometry is that hypothetically, it can be used to measure all those substances, which absorb light.